Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Corticosteroid binding in human artery.

H Sasano, N Watanabe, N Sasano

The Tohoku Journal of Experimental Medicine

|October 1, 1986

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Programmed death-1 ligands and tumor infiltrating T lymphocytes in primary and lymph node metastasis of esophageal cancer patients.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2018

Same author

Residual carcinoma cells after chemoradiotherapy for esophageal squamous cell carcinoma patients: striving toward appropriate judgment of biopsy.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2018

Same author

Aromatase expression in atypical ductal hyperplasia in women.

Breast cancer research and treatment·2017

Same author

A case of bilateral aldosterone-producing adenomas differentiated by segmental adrenal venous sampling for bilateral adrenal sparing surgery.

Journal of human hypertension·2015

Same author

Fibrosing cholestatic hepatitis developing within one month after living donor liver transplantation for chronic hepatitis C-related cirrhosis: a case report.

Transplantation proceedings·2014

Same author

Survival of HER2-positive primary breast cancer patients treated by neoadjuvant chemotherapy plus trastuzumab: a multicenter retrospective observational study (JBCRG-C03 study).

Breast cancer research and treatment·2014

Human arteries possess mineralocorticoid and glucocorticoid receptors, indicating that corticosteroids may directly impact arterial function. This finding is crucial for understanding steroid hormone effects on the cardiovascular system.

Area of Science:

Endocrinology
Vascular Biology
Molecular Pharmacology

Background:

Corticosteroids, including mineralocorticoids and glucocorticoids, play vital roles in physiological processes.
The presence and function of specific corticosteroid receptors in human arterial tissue remain incompletely understood.

Purpose of the Study:

To investigate the presence and characteristics of mineralocorticoid and glucocorticoid binding sites in human arteries.
To determine if human arteries possess functional corticosteroid receptors.

Main Methods:

Scatchard analysis was employed to quantify glucocorticoid binding in femoral and umbilical arteries.
Mineralocorticoid binding was analyzed in the inferior mesenteric artery using Scatchard analysis.
Polyacrylamide gel electrophoresis and sucrose density gradient centrifugation were used to characterize corticosteroid binding components in popliteal and femoral arteries, respectively.

Related Experiment Videos

Main Results:

Glucocorticoid receptors were identified in femoral and umbilical arteries with specific binding parameters (Kd and Bmax).
Mineralocorticoid binding in the inferior mesenteric artery suggested two distinct binding components with varying affinities and capacities.
Characterization techniques confirmed the presence of corticosteroid binding molecules in arterial cytosol, with sedimentation patterns indicative of receptor proteins.

Conclusions:

The study provides evidence for the existence of both mineralocorticoid and glucocorticoid receptors in human arterial tissue.
These findings suggest that corticosteroids may exert direct actions on human arteries, potentially influencing vascular function and health.