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FIBCD1 Binds Aspergillus fumigatus and Regulates Lung Epithelial Response to Cell Wall Components

Christine Schoeler Jepsen1, Lalit Kumar Dubey1,2, Kimmie B Colmorten1

  • 1Cancer and Inflammation Research, Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

Frontiers in Immunology
|October 4, 2018
PubMed

Insights

Fibrinogen C domain-containing protein 1 (FIBCD1) recognizes Aspergillus fumigatus cell walls in the human lung. FIBCD1 suppresses inflammatory responses and mucin production in lung epithelial cells.

Area of Science:

  • Immunology
  • Pulmonology
  • Microbiology

Background:

  • Aspergillus fumigatus is a common fungus causing lung diseases.
  • Fibrinogen C domain-containing protein 1 (FIBCD1) binds chitin and is found in the gut and salivary glands.
  • The role of FIBCD1 in the human lung and its interaction with A. fumigatus is not well understood.

Purpose of the Study:

  • To investigate FIBCD1 expression in human lung epithelium.
  • To determine if FIBCD1 recognizes Aspergillus fumigatus cell wall components.
  • To elucidate the functional role of FIBCD1 in modulating lung epithelial inflammatory responses.

Main Methods:

  • Immunohistochemistry to localize FIBCD1 in human lung tissue.
  • Cell-based assays using FIBCD1-transfected lung epithelial cells stimulated with A. fumigatus components.
  • Measurement of inflammatory mediators (e.g., IL-8) and mucin production.
  • Analysis of gene transcription related to inflammation and homeostasis.

Main Results:

  • FIBCD1 is expressed on the surface of human bronchial and alveolar epithelial cells.
  • FIBCD1 recognizes Aspergillus fumigatus cell wall polysaccharides independently of its chitin-binding site.
  • FIBCD1 suppresses IL-8 secretion, mucin production, and inflammatory gene expression in response to A. fumigatus stimulation.
  • FIBCD1 modulates IL-8 secretion induced by Toll-like receptors (TLR2, -4, and -5).

Conclusions:

  • FIBCD1 functions as a pattern recognition receptor in the human lung epithelium.
  • FIBCD1 recognizes complex polysaccharides of the Aspergillus fumigatus cell wall.
  • FIBCD1 plays a role in modulating the lung epithelial inflammatory response to fungal components by suppressing inflammatory mediators and mucins.
Keywords:
A549Aspergillus fumigatusFIBCD1IL-8epitheliumhumaninflammationlung

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