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Suppressing cell growth and inducing apoptosis by inhibiting miR‑23a‑5p in human bladder cancer

Yifan Li1, Jing Quan2, Xiang Pan2

  • 1Department of Urology, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu, Yangzhou, Jiangsu 225001, P.R. China.

Molecular Medicine Reports
|October 4, 2018
PubMed

Insights

MicroRNA-23a-5p (miR-23a-5p) is elevated in bladder cancer, promoting tumor cell growth and survival. Inhibiting miR-23a-5p suppressed proliferation and induced apoptosis, suggesting its oncogenic role and potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key regulators in cancer development.
  • miRNA-23a-5p (miR-23a-5p) is implicated in promoting cell growth and survival across various cancers.

Purpose of the Study:

  • To investigate the expression and functional role of miR-23a-5p in bladder cancer.
  • To determine if miR-23a-5p could serve as a potential therapeutic target for bladder cancer.

Main Methods:

  • Quantitative real-time PCR to measure miR-23a-5p expression in bladder cancer tissues versus normal tissues.
  • Cell proliferation assays (MTT), migration assays (wound scratch), and apoptosis assays (flow cytometry) were performed.
  • Transfection with miR-23a-5p inhibitors in bladder cancer cell lines (T24, SW780).

Main Results:

  • miR-23a-5p was significantly upregulated in human bladder cancer tissues compared to normal urothelial tissues.
  • Overexpression of miR-23a-5p promoted cell proliferation and migration, while inhibiting apoptosis.
  • Inhibition of miR-23a-5p led to decreased proliferation and increased apoptosis in bladder cancer cells.

Conclusions:

  • miR-23a-5p exhibits an oncogenic role in bladder cancer.
  • miR-23a-5p represents a potential therapeutic target for bladder cancer treatment.

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