Related Experiment Video
Updated: Feb 4, 2026

Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines
Published on: September 3, 2016
Comparison of cross-matching method for detection of DEA 7 blood incompatibility
Eva Spada1,2,3, Roberta Perego1,2,3, Luis Miguel Viñals Flórez1,2,3
1Veterinary Transfusion Research Laboratory (Spada, Perego, Baggiani, Proverbio) and Department of Veterinary Medicine (Dall'Ara), University of Milan, Milan, Italy.
Abstract:
We compared 3 major cross-match (XM) tests to identify dog erythrocyte antigen (DEA) 7 blood incompatibilities in dogs as a result of anti-DEA 7 antibodies: gel (GEL), standard tube (TUBE) agglutination, and immunochromatography strips (STRIP). Blood samples from 42 dogs were typed for DEA 7; 2 tested DEA 7-positive (DEA 7+). The 40 DEA 7-negative (DEA 7-) plasma samples were cross-matched against the 2 DEA 7+ and 3 DEA 7- red blood cell (RBC) samples by GEL to identify samples with anti-DEA 7 antibodies. Twenty DEA 7- plasma samples without and with anti-DEA 7 antibodies were cross-matched with samples of the 2 DEA 7+ RBCs in a double-blind fashion using the TUBE and STRIP XM methods. GEL results were used as the reference method for comparison. To determine relationships between results, 2 × 2 tables were used. Cohen kappa coefficient (κ) was calculated between results of GEL and the other 2 methods. With GEL, 21 of 40 XM tests were positive and 19 of 40 negative for anti-DEA 7 antibodies. The same results were obtained by TUBE, whereas only 1 of 40 XM tests was positive by STRIP. There was a statistically significant relationship between results of GEL and TUBE ( p < 0.000) with perfect agreement (κ = 1.000), but not between GEL and STRIP results ( p = 1.000) in which agreement was equivalent to chance (κ = 0.0453). The GEL and TUBE XM tests, but not STRIP, are useful methods for identification of DEA 7 incompatibilities caused by anti-DEA 7 antibodies.
More Related Videos
07:19Comparison of Kinetic Characteristics of Footwork during Stroke in Table Tennis: Cross-Step and Chasse Step
Published on: June 16, 2021
07:59Author Spotlight: Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons
Published on: June 9, 2023
Related Concept Videos
Crossing Over
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
Crossing Over
Sign Test for Matched Pairs
To conduct the sign test, we first calculate the differences in...
Renal Drug Clearance: Comparison Between Renal Excretion Methods
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
Monohybrid Crosses
Cross-Sectional Research