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Updated: Feb 4, 2026

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
Published on: April 28, 2015
Multilayer sensing platform: gold nanoparticles/prussian blue decorated graphite paper for NADH and H2O2 detection
1College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu 241000, P.R. China. kanxw@maill.ahnu.edu.cn.
Abstract:
In this study, an exfoliated graphite paper (EGP) was employed as a supported electrode for prussian blue (PB) and gold nanoparticles (AuNPs) modification to fabricate a novel electrochemical sensor. The scanning electron microscopy image showed the multilayer structure of the sensor with PB and AuNPs uniform coating. Due to the high surface area and good conductivity of the multilayer structure, as well as the conductivity of AuNPs, the prepared sensor exhibited an obvious improvement for sensitive detection of nicotinamide adenine dinucleotide (NADH) with a linear range of 5.0 × 10-7 mol L-1-1.0 × 10-3 mol L-1. Moreover, the modified PB film endowed the sensor with good electrocatalytic activity for hydrogen peroxide (H2O2) sensing. Under the optimal conditions, the linear range and limit of detection for H2O2 sensitive detection were 5.0 × 10-8 mol L-1-1.0 × 10-3 mol L-1 and 4.8 × 10-9 mol L-1, respectively. The sensor can also be used for detection in real samples with satisfactory results. The advantages of low-cost, easy modification, multilayer structure, and good conductivity made the EGP an alternative candidate for supported electrode materials in the preparation and application of various electrochemical sensors.
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