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Updated: Feb 4, 2026

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer
Published on: May 18, 2020
LncRNA LET function as a tumor suppressor in breast cancer development
1Department of Thyroid Breast Surgery, Liaocheng People's Hospital, Liaocheng, China. deji94741@163.com.
Long-chain non-coding RNA LET (lncRNA LET) is downregulated in breast cancer. Its overexpression inhibits cancer cell proliferation, invasion, and migration, while promoting apoptosis, suggesting it as a potential therapeutic target.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Long-chain non-coding RNAs (lncRNAs) play crucial roles in various biological processes.
- Aberrant lncRNA expression is implicated in the pathogenesis of numerous cancers, including breast cancer.
Purpose of the Study:
- To investigate the role of lncRNA LET in human breast cancer.
- To elucidate the underlying molecular mechanisms of lncRNA LET's function in breast cancer.
Main Methods:
- Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) for lncRNA expression analysis.
- MTT assay for cell proliferation.
- Flow cytometry for apoptosis assessment.
- Transwell and scratch assays for cell invasion and migration.
Main Results:
- lncRNA LET expression was significantly reduced in breast cancer tissues and cells.
- Overexpression of lncRNA LET inhibited breast cancer cell proliferation, invasion, and migration.
- lncRNA LET overexpression promoted cancer cell apoptosis and repressed epithelial-mesenchymal transition (EMT).
Conclusions:
- lncRNA LET acts as a tumor suppressor in breast cancer.
- lncRNA LET may serve as a novel diagnostic marker and therapeutic target for breast cancer treatment.
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