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Updated: Feb 4, 2026

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
Published on: April 26, 2018
Achieving greater efficiency and higher confidence in single-cell cloning by combining cell printing and plate
1Cell Culture, Pharma Technical Development, Genentech, Inc., 1 DNA Way South San Francisco, California, 94080.
Abstract:
In recent years, health authorities have increased emphasis on demonstrating that a cell line, which is used for the generation of biologics, is clonally derived. Within the past few years, single-cell manipulation technologies, especially microfluidic drop-on-demand dispensing, have gained increased attention in the biopharmaceutical industry. This work discusses the development and characterization of a single-cell printing workflow followed by plate imaging. By combining single-cell printing and plate imaging with manual image verification it is possible to, (1) dramatically reduce the number of microtiter plates needed during the single-cell cloning of clinical cell lines, as compared with a limiting-dilution single-cell cloning workflow, and therefore reduce the number of high-resolution images acquired and stored and (2) achieve >99.99% assurance that the cell lines derived from this workflow are clonally derived. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1454-1459, 2018.
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