Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.3K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.3K
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

5.2K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
5.2K
Molecules and Compounds02:38

Molecules and Compounds

68.8K
Atoms and Molecules
68.8K
Dynamic Equilibrium02:20

Dynamic Equilibrium

62.7K
A reversible chemical reaction represents a chemical process that proceeds in both forward (left to right) and reverse (right to left) directions. When the rates of the forward and reverse reactions are equal, the concentrations of the reactant and product species remain constant over time and the system is at equilibrium. A special double arrow is used to emphasize the reversible nature of the reaction. The relative concentrations of reactants and products in equilibrium systems vary greatly;...
62.7K
Chromatographic Resolution01:15

Chromatographic Resolution

2.1K
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Condensin I but not Condensin II is crucial for mitotic chromosome mechanics.

Nature communications·2026
Same author

Mechanistic basis for relaxation of DNA supercoils by human topoisomerase IIIα-RMI1-RMI2.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3.

bioRxiv : the preprint server for biology·2025
Same author

Calyculin A Induces Premature Chromosome Condensation and Chromatin Compaction in G<sub>1</sub>-Phase HeLa Cells without Histone H1 Phosphorylation.

bioRxiv : the preprint server for biology·2025
Same author

Sensory stimuli and cilium trafficking defects trigger the release of ciliary extracellular vesicles from multiple ciliary locations.

bioRxiv : the preprint server for biology·2025
Same author

Delivery of intraflagellar transport proteins to the ciliary base and assembly into trains.

Science advances·2025

Related Experiment Video

Updated: Feb 4, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

10.0K

Interplay between Ciliary Ultrastructure and IFT-Train Dynamics Revealed by Single-Molecule Super-resolution Imaging.

Felix Oswald1, Bram Prevo1, Seyda Acar1

  • 1Department of Physics and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, 1081HV, the Netherlands.

Cell Reports
|October 4, 2018
PubMed
Summary

Intraflagellar transport (IFT) dynamics are linked to ciliary structure. Impaired IFT in specific ciliary regions and kinesin-II mutants reveals a mutual interplay influencing ciliary organization and function.

Keywords:
ciliaintracellular transportintraflagellar transportmolecular motor proteinsmotor cooperationsingle-molecule fluorescence microscopysuper-resolution microscopy

More Related Videos

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.8K
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K

Related Experiment Videos

Last Updated: Feb 4, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

10.0K
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.8K
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K

Area of Science:

  • Cell Biology
  • Molecular Motors
  • Cilia Biology

Background:

  • Cilia are essential cellular structures maintained by intraflagellar transport (IFT).
  • The precise relationship between IFT dynamics and ciliary ultrastructure remains poorly understood.
  • Understanding this interplay is crucial for deciphering ciliary assembly and function.

Purpose of the Study:

  • To investigate the dynamic behavior of IFT trains within distinct ciliary subdomains.
  • To correlate IFT-train dynamics with ciliary ultrastructure using advanced imaging techniques.
  • To elucidate the role of kinesin-II in IFT dynamics and ciliary organization.

Main Methods:

  • Single-molecule super-resolution imaging of IFT components in living *C. elegans*.
  • Analysis of IFT-train dynamics in wild-type and kinesin-II mutant animals.
  • Correlation of dynamic IFT data with detailed ciliary ultrastructure.

Main Results:

  • IFT dynamics are impaired in the ciliary transition zone, leading to frequent pauses.
  • Distinct IFT-train turnaround dynamics were observed at the ciliary base and tip.
  • Kinesin-II deletion significantly altered IFT-train dynamics and ciliary structure, affecting IFT train-microtubule arrangements.
  • Super-resolution imaging revealed altered IFT train organization in kinesin-II mutants compared to wild-type.

Conclusions:

  • A complex, mutual interplay exists between ciliary ultrastructure and IFT-train dynamics.
  • Physical cues within ciliary subdomains significantly influence IFT dynamics.
  • IFT motor proteins like kinesin-II play a critical role in both IFT and maintaining ciliary architecture.