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Related Experiment Videos

Human B lymphocytes in giemsa stained preparations.

C R Taylor, I L Gordon, A Rembaum

    Journal of Immunological Methods
    |January 1, 1977
    PubMed
    Summary

    A novel mixed antiglobulin method allows simultaneous analysis of cell surface immunoglobulin and cell details. This technique revealed significant changes in immunoglobulin-bearing cells after incubation or rosette enrichment.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • Accurate characterization of cell surface markers is crucial for understanding immune cell function.
    • Existing methods may not allow simultaneous assessment of cell surface immunoglobulin and detailed morphology.

    Purpose of the Study:

    • To develop and validate a mixed antiglobulin procedure for simultaneous analysis of cell surface immunoglobulin and cytological features.
    • To investigate the impact of incubation and cell enrichment on immunoglobulin expression on various leukocyte populations.

    Main Methods:

    • Development of a mixed antiglobulin staining technique.
    • Application of Giemsa staining for detailed cytological examination.
    • Incubation of lymphocytes at 37°C and sheep erythrocyte rosette enrichment procedures.

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  • Quantification of surface immunoglobulin-bearing lymphocytes, monocytes, and granulocytes.

    • Main Results:

    • The developed method enables concurrent evaluation of cell surface immunoglobulin and Giemsa-stained cytological characteristics.
    • Significant alterations in the percentage of surface immunoglobulin-positive lymphocytes, monocytes, and granulocytes were observed.
    • These changes were evident after lymphocyte incubation at 37°C and following sheep erythrocyte rosette enrichment.

    Conclusions:

    • The mixed antiglobulin procedure is effective for simultaneous immunophenotyping and cytological analysis.
    • Cellular activation or manipulation, such as incubation or rosette formation, significantly affects surface immunoglobulin expression.
    • This technique offers a valuable tool for studying dynamic changes in leukocyte surface markers.