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In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Published on: November 27, 2014
The complete genome sequence analysis of West Nile virus strains isolated in Slovakia (central Europe)
Petra Drzewnioková1, Luisa Barzon2,3, Elisa Franchin2,3
1Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia.
Abstract:
The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3, E159 and E231) and in non-structural (NS1109, NS5259, NS5310 and NS5600) proteins. Observed amino acid substitutions Phe3 and Ser231 were common only within the lineage VII Koutango strain isolated from Rhipicephalus guilhoni tick in Senegal. Further research could reveal whether these substitutions influence the biological properties of WNV, including virulence and neuroinvasiveness.
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