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Updated: Feb 4, 2026

Platelet-Rich Plasma Lysate for Treatment of Eye Surface Diseases
Published on: August 2, 2022
Platelet-rich plasma lyophilization enables growth factor preservation and functionality when compared with fresh
Letícia Queiroz da Silva1, Silmara Aparecida de Lima Montalvão1, Amauri da Silva Justo-Junior2
1Hemocentro, Haemostasis Laboratory, State University of Campinas-UNICAMP. 13083-970, Campinas, São Paulo, Brazil.
Aims:
To compare levels and activity of the growth factors between fresh and lyophilized platelet-rich plasma (PRP).
Methods:
Analysis of platelet concentration using fibroblast and human umbilical vein endothelial cell cultures were compared between fresh and lyophilized PRP obtained from peripheral blood.
Results:
After lyophilization, 54% of platelets were intact whereas the fresh showed no aggregation with agonists (levels under 20%). The concentration of growth factors (VEGF, EGF, TGF-β and PDGF) in both products were similar. Fresh and lyophilized PRPs induced proliferation in the fibroblasts at 24 h (0.303 vs 0.300, respectively).
Conclusion:
Lyophilized PRP appears to be an alternative to fresh PRP and the results evidenced the role of growth factors as a key element in the activity of this product.
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