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Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles

T S Gerashchenko1, E V Denisov2, N M Novikov3

  • 1Laboratory of Molecular Oncology and Immunology, Cancer Research Institute, Tomsk National Research Medical Center, Tomsk 634050, Russia.

Experimental Oncology
|October 5, 2018
PubMed
Abstract

Insights

Breast cancer

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Breast cancer exhibits diverse morphological structures, including tubular, alveolar, solid, trabecular, and discrete forms.
  • Understanding the molecular basis of drug resistance in these distinct structures is crucial for effective cancer treatment.

Purpose of the Study:

  • To identify gene expression profiles associated with drug resistance in different breast cancer morphological structures.
  • To elucidate how specific gene expression patterns in these structures influence sensitivity or resistance to anticancer drugs.

Main Methods:

  • Whole-transcriptome profiling using laser microdissection-assisted microarrays and quantitative real-time PCR (qRT-PCR).
  • Analysis of gene expression in ten patients with luminal breast cancer across various morphological subtypes.
  • Identification and validation of differentially expressed genes related to drug response.

Main Results:

  • Identified 27 differentially expressed genes (p < 0.05) involved in drug uptake, efflux, targeting, detoxification, cell cycle, apoptosis, and DNA repair.
  • Each morphological structure displayed a unique gene expression profile linked to drug resistance and sensitivity.
  • Alveolar structures showed the highest expression of these genes (19/27), significantly associated with drug resistance.

Conclusions:

  • Distinct morphological structures within breast cancer possess individual gene expression profiles that dictate drug resistance.
  • Targeting specific gene expression patterns in different structures may offer novel therapeutic strategies for breast cancer.

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