Ploidy Has Minimal Effect on Hypoxia Tolerance at High Temperature in Rainbow Trout (Oncorhynchus mykiss)

Polyploidy is an important driver of evolutionary change (generally via tetraploidy) and also serves a practical role in aquaculture and fisheries management (via triploidy). Fundamental changes in cell size and number that accompany polyploidy are predicted to affect cellular and whole-animal physiology due to constraints placed on surface-mediated processes at the cellular level, potentially altering environmental tolerances and optima. The aim of this study was to determine whether the documented reduction in thermal tolerance of aquatic polyploids is a result of their being less hypoxia tolerant. This was assessed by holding diploid and triploid rainbow trout for 1 h above their thermal optima in separate trials at eight temperatures between 20° and 27°C and then rapidly reducing the oxygen tension (Po₂) of the water and determining the nonlethal Po₂ at which fish lost equilibrium. As expected, there was a highly significant ([Formula: see text]) effect of temperature on Po₂ at loss of equilibrium. Although there was also a significant ([Formula: see text]) effect of ploidy on Po₂ at loss of equilibrium, with triploid values higher than diploid, post hoc analyses showed no significant effect of ploidy at any specific temperature. Oxygen availability alone therefore does not appear to play a major role in determining the thermal tolerance of polyploids.