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Related Experiment Video

Updated: Feb 4, 2026

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
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Exosome-transmitted long non-coding RNA PTENP1 suppresses bladder cancer progression.

Rui Zheng1,2, Mulong Du1,2,3, Xiaowei Wang1,2

  • 1Department of Environmental Genomics, School of Public Health, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, 101 Longmian Avenue, Jiangning District, Nanjing, 211166, China.

Molecular Cancer
|October 5, 2018
PubMed
Summary

Exosomal long non-coding RNA PTENP1 is reduced in bladder cancer (BC). This exosomal PTENP1 can be detected in plasma and suppresses BC progression by inhibiting cell invasion and migration.

Keywords:
BiomarkerBladder cancerExosomesPTENP1Progression

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Extracellular communication via exosomes and long non-coding RNAs (lncRNAs) is crucial in the tumor microenvironment.
  • The specific role of exosomal lncRNA PTENP1 in bladder cancer (BC) progression and its diagnostic potential were previously unclear.

Purpose of the Study:

  • To investigate the expression and function of exosomal PTENP1 in bladder cancer.
  • To evaluate exosomal PTENP1 as a potential diagnostic biomarker for BC.

Main Methods:

  • Detected PTENP1 expression in BC tissues and plasma exosomes from patients and healthy controls.
  • Assessed diagnostic accuracy using receiver operating characteristic (ROC) curve analysis.
  • Performed in vitro cell assays and in vivo animal experiments to determine the functional impact of exosomal PTENP1.

Main Results:

  • PTENP1 expression was significantly lower in BC tissues and plasma exosomes compared to controls.
  • Exosomal PTENP1 demonstrated diagnostic potential for BC with an AUC of 0.743.
  • Transfer of exosomal PTENP1 from normal to BC cells inhibited BC cell proliferation, invasion, migration, and tumor growth in vivo, partly by regulating PTEN expression via microRNA-17.

Conclusions:

  • Exosomal PTENP1 is a promising biomarker for the clinical detection of bladder cancer.
  • Exosomal PTENP1 transferred from normal cells to BC cells can suppress BC progression both in vitro and in vivo.
  • Exosomal PTENP1 plays a role in normal-cell-to-bladder-cell communication during BC carcinogenesis.