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Adriamycin inhibits embryonic development in zebrafish through downregulation of Kruppel-like factor4

I Syeda Thabassum Akthar1, P B Tirupathi Pichiah2, Sankarganesh Arunachalam3

  • 1Department of Integrative Biology, School of Biosciences and Technology, VIT, Vellore, Tamilnadu, India.

Journal of Biochemical and Molecular Toxicology
|October 5, 2018
PubMed

Insights

Adriamycin, an anticancer drug, disrupts zebrafish embryonic development by altering Kruppel-like factor 4 (Klf4) gene expression. This impacts hatching gland cell differentiation and hatching success in a dose-dependent manner.

Area of Science:

  • Developmental Biology
  • Pharmacology
  • Genetics

Background:

  • Adriamycin is a widely used anticancer drug.
  • Anticancer drugs can affect oncogenes and regulatory molecules crucial for development.
  • Kruppel-like factor 4 (Klf4) is a key factor in cellular pluripotency, growth, proliferation, and differentiation.

Purpose of the Study:

  • To investigate the impact of adriamycin on embryonic development.
  • To examine the effect of adriamycin on Kruppel-like factor 4 (Klf4) expression in zebrafish embryos.

Main Methods:

  • Zebrafish embryos were used as a model system.
  • The study analyzed the dose-dependent effects of adriamycin.
  • Gene expression levels of Klf4 and hatching markers (cathepsin L, cyclase-associated protein1) were assessed.

Main Results:

  • Adriamycin exposure altered Klf4 gene expression in a dose-dependent manner.
  • The observed changes in Klf4 expression correlated with adverse effects on embryonic hatching.
  • Expression of cathepsin L and cyclase-associated protein1, also hatching markers, were similarly affected.

Conclusions:

  • Adriamycin interferes with embryonic development in zebrafish.
  • Klf4 plays a critical role in zebrafish embryogenesis, specifically in hatching gland cell differentiation.
  • Adriamycin's detrimental effects on hatching are linked to its modulation of Klf4 and other hatching-related genes.
Keywords:
Kruppel-like factor4 (Klf4)adriamycinembryogenesishatching

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