Ergothioneine production with Aspergillus oryzae.
Shun Takusagawa1, Yasuharu Satoh2, Iwao Ohtsu3
1a Graduate School of Chemical Science and Engineering , Hokkaido University , Sapporo , Hokkaido , Japan.
Bioscience, Biotechnology, and Biochemistry
|October 6, 2018
Summary
Researchers developed a novel method for producing ergothioneine (ERG) using Aspergillus oryzae fermentation. This enhanced production yielded 20 times more ERG compared to the wild type, establishing a practical supply for this antioxidant.
Area of Science:
- Biotechnology
- Microbial Fermentation
- Metabolic Engineering
Background:
- Ergothioneine (ERG) is a potent antioxidant with potential health benefits.
- Current methods for ergothioneine production are often inefficient or impractical.
- Aspergillus oryzae is a safe, food-grade fungus suitable for industrial fermentation.
Purpose of the Study:
- To develop a reliable and scalable method for ergothioneine production.
- To enhance ergothioneine yield using genetic engineering in Aspergillus oryzae.
Main Methods:
- Heterologous overexpression of ergothioneine biosynthesis genes (egt-1 and egt-2) from Neurospora crassa in Aspergillus oryzae.
- Fermentative production of ergothioneine using engineered Aspergillus oryzae strains.
- Quantification of ergothioneine production using LC-ESI-MS.
Main Results:
- Achieved significant ergothioneine production of 231.0 mg/kg of media.
- Demonstrated a 20-fold increase in ergothioneine yield compared to the wild-type strain.
- Successfully established a fermentative production system for ergothioneine.
Conclusions:
- The engineered Aspergillus oryzae strain provides a highly efficient system for ergothioneine production.
- This study establishes a practical and scalable method for obtaining ergothioneine.
- The developed fermentation process holds promise for industrial applications of ergothioneine.
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