Ergothioneine production with Aspergillus oryzae
Shun Takusagawa1, Yasuharu Satoh2, Iwao Ohtsu3
1a Graduate School of Chemical Science and Engineering , Hokkaido University , Sapporo , Hokkaido , Japan.
Abstract:
To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine; l-Cys: l-cysteine; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry.
More Related Videos
10:51Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles
Published on: March 15, 2012
09:44RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
Published on: December 21, 2015
Related Concept Videos
Scalar Product (Dot Product)
The scalar product of two vectors is obtained by multiplying...
Vector Product (Cross Product)
Consider the cross product of two vectors. Imagine rotating the first vector about...
Primary Production
Production Efficiency
The Dot Product
Dot Product
In engineering, the dot product of any two vectors is the product of the magnitudes of the vectors and the cosine of the angle between them. It is denoted by a dot symbol between the two vectors.
Consider a vehicle pulling an object along the ground using a rope. If the rope makes an angle with the horizontal axis, the work done can be calculated using the dot product of the force applied and the object's displacement.
The dot...
