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A Practical Guide to Phylogenetics for Nonexperts
Published on: February 5, 2014
Characterization of the complete mitochondrial genome of Simulium (Byssodon) maculatum (Diptera: Simuliidae) and its
1Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, China.
Abstract:
The mitochondrial (mt) genome of the black fly Simulium (Byssodon) maculatum, a pest of great importance for both humans and livestock, is sequenced and annotated for the first time. The genome structure, gene order and codon usage are typical among Diptera mt genomes. The mt genome is circular and 15,799 bp in length with 13 protein coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a control region (CR), and with weakly positive AT-skew (0.02) and negative GC-skew (-0.12). Phylogenetic relationships of 16 species representing five families of Culicomorpha and two outgroups, based on mt genome data, were analyzed using both Maximum Likelihood and Bayesian methods. The monophyly of Culicomorpha is well supported, while Chironomoidea is indicated as a paraphyletic group. The well supported monophyletic Simuliidae is the sister group to Culicidae.
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