Related Experiment Video

Updated: Feb 4, 2026

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight
08:03

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight

Published on: May 31, 2022

5.6K

Structural insights into the recognition of phosphorylated Hop1 by Mek1

Changlin Xie1, Chao He2, Yiyang Jiang3

  • 1High Magnetic Field Laboratory, Chinese Academy of Sciences, 50 Shushanhu Road, Hefei, Anhui 230031, People's Republic of China.

Acta Crystallographica. Section D, Structural Biology
|October 6, 2018
PubMed

Insights

Researchers elucidated how the Mek1 kinase recognizes phosphorylated Hop1 protein during meiosis. Crystal structures reveal specific hydrophobic residues crucial for this interaction, enhancing understanding of recombination regulation in yeast.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Structural Biology

Background:

  • Mek1 is a meiosis-specific kinase regulating interhomolog recombination in Saccharomyces cerevisiae.
  • Mek1 activation depends on phosphorylated Hop1 (pT318), recognized by the Mek1 FHA domain.

Purpose of the Study:

  • To determine the structural basis for Mek1 FHA domain recognition of phosphorylated Hop1.
  • To investigate Mek1 FHA domain interactions in both S. cerevisiae and Schizosaccharomyces pombe.

Main Methods:

  • X-ray crystallography was used to obtain structures of the Mek1 FHA domain.
  • Complex structures were determined with a Hop1 pT318 peptide.

Main Results:

  • Crystal structures revealed the Mek1 FHA domain in apo and peptide-bound states.
  • Hydrophobic residues Phe320 and Val321 in Hop1 peptide are key for Mek1 FHA recognition.
  • In S. pombe, Mek1 FHA binds phosphorylated sites in both Mek1 and Hop1 SQ/TQ cluster domains.

Conclusions:

  • The study provides structural insights into Mek1-Hop1 interaction specificity in S. cerevisiae.
  • Understanding these interactions aids in comprehending Mek1 function and its binding targets in different yeast species.
Keywords:
FHA domainHop1Mek1meiotic recombinationphosphointeraction

More Related Videos

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.2K
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.2K

Related Concept Videos

Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
53.9K
Structures of Solids02:22

Structures of Solids

Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
17.8K
Structural Isomerism02:34

Structural Isomerism

Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.7K
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
98.9K
Viral Structure00:56

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.6K
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.6K