Suppressing inflammation and enhancing osteogenesis using novel CS-EC@Ca microcapsules

The aim of this study was to investigate the suppression of inflammation and enhancement of osteogenesis using chitosan-coated calcium hydroxide-loaded microcapsules (CS-EC@Ca microcapsules) in vivo. Circular defects were created in the mandibular bones of rabbits and filled with Ca(OH)₂ , Bio-oss, or CS-EC@Ca microcapsules, and rabbits without drug implantation served as the controls. Lipopolysaccharides were injected in situ daily in all groups for 7 days. Mandibular bones were investigated at 4 and 12 weeks after surgery using micro-CT, histological observations, and real-time PCR analysis. At the postoperation, there was more substantial nascent bone in the microcapsule and Bio-oss groups than in the control group. The recovery of the rabbits in the Ca(OH)₂ group was slower than the control group, as determined using micro-CT and histological staining. Osteocalcin and collagen type I production was not significantly different between the microcapsule and Bio-oss groups (p > 0.05), but the expression levels of the two molecules were significantly increased compared to the control and Ca(OH)₂ groups at postoperation (p < 0.05). The mRNA transcript levels of inflammatory factors in the microcapsule group had the most reduced expression of IL-6 and TNF-α (p < 0.05). The microcapsules significantly reduced inflammation and promoted osteogenesis in this rabbit model of inflammatory bone destruction. Our findings indicate that CS-EC@Ca microcapsules hold potential for use in apical periodontitis treatment. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3222-3230, 2018.