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Updated: Feb 4, 2026

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
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High-Throughput DNA Plasmid Transfection Using Acoustic Droplet Ejection Technology.

Béatrice Colin1, Benoit Deprez1, Cyril Couturier1

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Summary

This study demonstrates the Labcyte Echo 550 device for efficient plasmid DNA transfection, achieving 90% efficiency in HeLa cells. The automated protocol optimizes transfection parameters, enabling rapid assay development for various applications.

Keywords:
acoustic droplet ejectionhigh throughputliquid handlingmammalian cellstransfection

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics Engineering

Background:

  • The Labcyte Echo acoustic liquid handler is known for precise droplet dispensing in various biological assays.
  • Previous applications include quantitative PCR, protein crystallization, drug screening, and siRNA transfection.
  • However, its use in plasmid DNA transfection assays has not been previously reported.

Purpose of the Study:

  • To evaluate the Echo 550 device for plasmid DNA transfection in 384-well plates.
  • To simultaneously optimize key transfection parameters: reagent dilution, DNA amount, and DNA concentration.
  • To develop a fast, reliable, and automated protocol for plasmid DNA transfection.

Main Methods:

  • Utilized the Echo 550 acoustic liquid handler for precise droplet transfer.
  • Optimized transfection reagent dilution, DNA amount, and starting DNA concentration.
  • Developed and tested a four-step transfection protocol on HeLa cells.

Main Results:

  • Achieved up to 90% transfection efficiency in HeLa cells.
  • Reached nearly 100% co-expression in co-transfection experiments.
  • Demonstrated a fast, reliable, and automated transfection protocol.

Conclusions:

  • The Echo 550 device is effective for high-throughput plasmid DNA transfection.
  • The developed protocol allows rapid optimization of transfection settings for diverse cell types.
  • This technology facilitates new applications like ORFeome protein expression and CRISPR-Cas9 screening.