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Asparagine alters action potential parameters in single plant cell
Indre Lapeikaite1, Ugne Dragunaite2, Vilmantas Pupkis2
1Institute of Biosciences, Life Sciences Centre, Vilnius University, Sauletekio Ave. 7, LT-10257, Vilnius, Lithuania. pinivija@gmail.com.
Protoplasma
|October 7, 2018
Summary
The amino acid L-asparagine alters electrical signaling in single plant cells. It affects action potential parameters in a dose-dependent manner, influencing cell excitability.
Area of Science:
- Plant physiology
- Cellular electrophysiology
- Biochemistry
Background:
- Electrical signaling is crucial for plant cell function.
- Exogenous amino acids can modulate cellular processes.
- Nitellopsis obtusa serves as a model for studying plant cell electrophysiology.
Purpose of the Study:
- To investigate the effect of L-asparagine on the electrical signaling of single Nitellopsis obtusa cells.
- To determine the dose-dependent response of action potential parameters to L-asparagine.
Main Methods:
- Utilized the glass-microelectrode technique.
- Employed both current-clamp and voltage-clamp modes.
- Exposed single Nitellopsis obtusa cells to varying concentrations of L-asparagine (0.1 mM and 1 mM) for 30 minutes.
Main Results:
- L-asparagine exposure altered electrically stimulated action potential (AP) parameters.
- Observed a dose-dependent effect, including increased AP amplitude and hyperpolarized AP threshold potential (Eth).
- Prolonged action potential repolarization and increased maximum chloride (Cl-) efflux amplitude were noted, along with extended activation and inactivation durations.
Conclusions:
- L-asparagine significantly impacts the excitability and action potential characteristics of individual plant cells.
- Findings contribute to understanding the role of exogenous amino acids in plant electrical signaling.
- Provides new insights into the specific effects of L-asparagine on single-cell AP dynamics.
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