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Updated: Feb 4, 2026

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Published on: December 22, 2020
Asparagine alters action potential parameters in single plant cell
Indre Lapeikaite1, Ugne Dragunaite2, Vilmantas Pupkis2
1Institute of Biosciences, Life Sciences Centre, Vilnius University, Sauletekio Ave. 7, LT-10257, Vilnius, Lithuania. pinivija@gmail.com.
Abstract:
Effect of amino acid L-asparagine on electrical signalling of single Nitellopsis obtusa (Characeaen) cell was investigated using glass-microelectrode technique in current-clamp and voltage-clamp modes. Cell exposure for 30 min to 0.1 mM and 1 mM of asparagine resulted in changes of electrically stimulated action potential (AP) parameters in comparison to standard conditions. Results indicate that asparagine acts in dose-dependent manner: increases AP amplitude by hyperpolarizing AP threshold potential (Eth), prolongs action potential repolarization, increases maximum Cl- efflux amplitude along with the increase of activation and inactivation durations. Presented findings provide new aspects of exogenous amino acids' effect on plants' electrical signalling with emphasis on separate single plant cell excitability and AP characteristics.
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