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Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications
Published on: February 6, 2016
Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe
Fangmei Zhang1,2, Pinyi Ma3, Xinyu Deng1
1College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, People's Republic of China.
Abstract:
Glutathione-capped water-soluble CuInS/ZnS quantum dots (QDs) were prepared by a microwave-assisted method. Their fluorescence, with excitation/emission peaks at 380/570 nm, is found to be quenched by hydrogen peroxide (H2O2) that is produced by the uricase catalyzed oxidation of uric acid (UA) and oxygen. The findings are used in a quenchometric method for the determination of UA. The effects of different ligands on the QDs, of pH value, buffers, enzyme ratio and reaction time were optimized. The detection limit for UA is 50 nM which is lower than other QD-based method, and the detection ranges extends from 0.25-4.0 μM. The assay is simple and sensitive, and no further modification of the QDs is required. Graphical abstract ᅟ.
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