Citrate analysis using capillary electrophoresis and complexation with Eu3+-tetracycline
Douglas B Craig1, Jennifer R Lischynski2, Isabela C C Cardoso2
1Department of Chemistry, University of Winnipeg, 599 Portage Avenue, Winnipeg, MB, R3B 2G3, Canada. d.craig@uwinnipeg.ca.
Summary
A new sensitive assay for citrate detection was developed using capillary electrophoresis and laser-induced luminescence. This method accurately quantifies citrate in various products and enables enzyme assays.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Citrate is a crucial metabolite in biological systems.
- Accurate quantification of citrate is essential for various applications.
Purpose of the Study:
- To develop a sensitive and rapid assay for citrate detection.
- To apply the assay for analyzing citrate in real-world samples and enzyme studies.
Main Methods:
- Developed a capillary electrophoresis (CE) method with post-column laser-induced luminescence detection.
- Utilized a Europium(III)-tetracycline complex for sensitive citrate detection.
- Employed a sheath-flow cuvette for efficient detection.
Main Results:
- Achieved linear signal response for citrate concentrations from 10 μM to 200 nM.
- Demonstrated high sensitivity with detection of 64 amoles of citrate.
- Reported rapid separation (<90 s) and a total run time of 5 min.
Conclusions:
- The developed CE-LIL assay provides a sensitive, rapid, and efficient method for citrate quantification.
- The assay is applicable to agricultural and medicinal products.
- The method facilitates the development of assays for enzymes like citrate synthase.
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