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Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
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Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

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Soluble CD40L and CD62P levels differ in single-donor apheresis platelet concentrates and buffy coat-derived pooled

Caroline Sut1,2, Sofiane Tariket1,2, Chaker Aloui1,2

  • 1Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Etienne, France.

Transfusion
|October 7, 2018
PubMed

Insights

Platelet concentrates (PCs) show inflammatory marker increases during storage, with pooled PCs (PPCs) rising more than single-donor PCs (SDA-PCs). Platelet additive solutions (PASs) impact marker release, highlighting the importance of processing and storage conditions.

Area of Science:

  • Transfusion Medicine
  • Hematology
  • Biochemistry

Background:

  • Platelet storage lesions (PSLs) are biochemical and structural changes affecting platelet concentrates (PCs).
  • Platelet additive solutions (PASs) can mitigate PSLs by optimizing storage conditions.
  • Understanding PSL development is crucial for improving platelet transfusion safety and efficacy.

Purpose of the Study:

  • To investigate the release of inflammatory markers soluble CD40 ligand (sCD40L) and soluble P-selectin (sCD62P) in platelet concentrates.
  • To compare these releases between single-donor apheresis platelet concentrates (SDA-PCs) and buffy coat-derived pooled platelet concentrates (PPCs).
  • To assess the impact of storage duration and platelet additive solutions (PASs) on marker release.

Main Methods:

  • Analyzed 9089 samples of SDA-PCs and PPCs processed via various methods.
  • Quantified sCD40L and sCD62P levels in PC supernatants using Luminex technology.
  • Measured marker levels immediately post-processing and at the time of delivery after storage.

Main Results:

  • SDA-PCs showed higher activation than PPCs pre-storage.
  • PPCs exhibited greater increases in inflammatory markers during storage compared to SDA-PCs.
  • Platelet additive solution-D (PAS-D) reduced sCD62P but increased sCD40L secretion in SDA-PCs compared to PAS-E.

Conclusions:

  • PC processing and storage significantly influence inflammatory marker release.
  • The type of PAS used affects the secretion profile of inflammatory markers.
  • Further clinical studies are needed to correlate these findings with patient outcomes.
Abstract

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