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Published on: September 7, 2018
A promising laccase immobilization approach for Bisphenol A removal from aqueous solutions
Fatiha Lassouane1, Hamid Aït-Amar2, Saïd Amrani3
1Université des Sciences et de la Technologie Houari Boumediene (USTHB), Laboratoire des Sciences du Génie des Procédés Industriels, Faculté de Génie Mécanique et de Génie des Procédés, BP 32, El-Alia 16111, Algiers, Algeria; Centre de Développement des Energies Renouvelables, CDER, 16340 Algiers, Algeria.
Abstract:
The immobilization of crude laccase from Trametes pubescens by glutaraldehyde crosslinking prior to entrapment into Ca-alginate beads increased the immobilization yield by 30% and reduced the leaking by 7-fold compared to the immobilization with no crosslinking. The performance of the newly developed biocatalyst to degrade Bisphenol A (BPA) from aqueous solutions was tested. Thus, operating at optimal conditions (i.e. pH 5, 30 °C, 20 mg L-1 BPA and 1500 U L-1 laccase), a BPA removal higher than 99% in 2 h was achieved. This value is higher than those reported to date for BPA removal by immobilized laccases. In addition, the biocatalyst was able to remove BPA in 10 successive batches with an efficiency higher than 70% at the end of the last batch. BPA adsorption on the alginate beads was negligible, therefore BPA removal was only due to laccase action. Moreover, Fourier-transform Infrared (FTIR) spectroscopy suggested BPA transformation by laccase.
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