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Updated: Feb 4, 2026

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
Published on: November 17, 2017
Stability of plasma proteins and factors in Chinese universal pooled plasma
Liguo Zhu1, Liping Sun1, Fengyan Fan1
11 Department of Blood Transfusion, Chinese PLA General Hospital, Beijing, China.
Objective:
This study aimed to determine the precision dose of Chinese universal pooled plasma (CUPP) developed by our laboratory, and the stability of plasma proteins and factors.
Methods:
A total of 100 single fresh-frozen plasma (FFP) units were selected to test plasma proteins, including total protein, albumin, fibrinogen, factor V, factor VIII, antithrombin-III, and protein C. Different pooling protocols with 20, 40, 60, 80, and 100 units were used to optimize the number of pooled units. The pooled plasma was then used to further evaluate the optimal storage conditions and duration at 22°C, 4°C, and -20°C.
Results:
There were considerable differences in plasma protein levels among single units of FFP. After different pooling protocols, the mean value of plasma proteins did not significantly change. However, with a larger number of pooled samples, plasma proteins were more stable with a smaller standard deviation. Acceptable storage for CUPP was achieved with storage for 1 day at 22°C, 4 days at 4°C, and 3 months at -20°C.
Conclusion:
A uniform level of plasma proteins and factors in CUPP appears to support establishment of a precise dose of plasma.
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