Generation of controllable gaseous H₂S concentrations using microfluidics

Area of Science:

• Biochemistry
• Cell Biology
• Bioengineering

Background:

• Hydrogen sulfide (H₂S) is a crucial signaling molecule, but its instability complicates in vitro research.
• Identifying H₂S targets and functions is hindered by difficulties in maintaining stable concentrations.

Purpose of the Study:

• To develop a microfluidic system for precise and stable delivery of hydrogen sulfide (H₂S) to cells.
• To overcome limitations of bolus H₂S delivery methods in biological studies.

Main Methods:

• A microfluidic device utilizing a gas-permeable polydimethylsiloxane membrane was engineered.
• Hydrogen sulfide gas was generated from a sodium hydrosulfide solution perfused beneath the membrane.
• Electrochemical sensors monitored H₂S concentrations, controlled by donor concentration and flow rate.

Main Results:

• The system provided stable H₂S concentrations for up to 5 hours.
• H₂S levels were precisely controlled by adjusting sodium hydrosulfide concentration and solution flow rate.
• Variable H₂S concentration profiles were achieved by altering flow rates.

Conclusions:

• This microfluidic system offers a novel method for controlled H₂S delivery in in vitro and ex vivo research.
• The technology facilitates the identification of novel biological processes and cellular mechanisms regulated by H₂S.