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Updated: Feb 4, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

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Detection of MAPK/ERK pathway proteins and KRAS mutations in adenomatoid odontogenic tumors

Ronell Bologna-Molina1, Ikuko Ogawa2, Adalberto Mosqueda-Taylor3

  • 1Molecular Pathology Area, Faculty of Dentistry, Universidad de la República, Montevideo, Uruguay.

Oral Diseases
|October 9, 2018
PubMed
Abstract

Insights

KRAS mutations were found in adenomatoid odontogenic tumors, alongside MAPK/ERK pathway proteins, suggesting a role in tumor development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pathology

Background:

  • Adenomatoid odontogenic tumors (AOTs) are rare benign neoplasms.
  • The molecular mechanisms underlying AOT development are not fully understood.
  • Investigating genetic mutations and signaling pathways can elucidate AOT pathogenesis.

Purpose of the Study:

  • To determine the frequency of KRAS mutations in AOTs.
  • To assess the presence of MAPK/ERK signaling pathway proteins in AOTs.
  • To explore the association between KRAS mutations and MAPK/ERK pathway proteins in AOTs.

Main Methods:

  • Analysis of paraffin-embedded AOT tissue samples (n=9).
  • Genomic DNA extraction and next-generation sequencing for mutation analysis.
  • Luminex assay for RAS family mutations and immunohistochemistry for pathway proteins (KRAS, CRAF, BRAF, EGFR, ERK, MEK, BRAFV600E).

Main Results:

  • KRAS mutations (G12D, G12V, G12R) were detected in tumor cells.
  • All cases showed positive expression for EGFR, KRAS, BRAF, and CRAF.
  • Most cases exhibited positive expression for MEK and ERK, with some exceptions.

Conclusions:

  • KRAS mutations at codon 12 are present in AOTs.
  • MAPK/ERK pathway proteins are present in AOTs.
  • These findings suggest a potential association between KRAS mutations, MAPK/ERK pathway activation, and AOT tumorigenesis.
Keywords:
ERK pathwayKRASMAPKadenomatoid odontogenic tumormutationnext-generation sequencing

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