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Updated: Feb 4, 2026

Induction and Assessment of Levodopa-induced Dyskinesias in a Rat Model of Parkinson's Disease
Published on: October 14, 2021
Sensitive LC-MS/MS method for quantitation of levodopa and carbidopa in plasma: application to a pharmacokinetic
Mônica S Ferreira1, Marcelo G Davanço2, Giovanni T Pepi1
1Centro Avançado de Estudos e Pesquisas - CAEP, P.O. Box: 245, Campinas, SP 13087-567, Brazil.
Aim:
The combination of levodopa with carbidopa has been used for treatment of Parkinson's disease being an important therapy in dopamine level control in the brain. Both are very polar compounds becoming a challenge for analysis by LC-MS/MS.
Materials & Methods:
In this work, it was developed and validated a sensitive bioanalytical method by UHPLC-MS/MS for simultaneous levodopa and carbidopa quantification in human plasma using a fast protein precipitation method. Moreover, the bioanalytical method was applied to a pharmacokinetic study in healthy volunteers.
Results/Conclusion:
The results demonstrated a sensitive and adequate method for application to pharmacokinetic/bioequivalence studies.
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