Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins
Suchismita Panda1, Meenakshi Setia1, Navjot Kaur1
1National Centre for Cell Science (NCCS), Savitribai Phule Pune University Campus, Pune, India.
Plos Biology
|October 9, 2018
Summary
A novel long noncoding RNA, Genomic Instability Inducing RNA (Ginir), promotes cancer by disrupting the interaction between Cep112 and Brca1 proteins, leading to mitotic abnormalities. Inhibiting Ginir restores genomic stability.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Long noncoding RNAs (lncRNAs) are crucial regulators of cellular processes.
- Dysregulation of lncRNAs is implicated in various diseases, including cancer.
- The functional roles of many lncRNAs remain largely uncharacterized.
Purpose of the Study:
- To functionally characterize a novel pair of complementary lncRNAs, Ginir and Giniras, from mouse cells.
- To elucidate the mechanism by which Ginir contributes to malignant transformation.
- To investigate the role of Ginir in regulating protein interactions critical for genomic stability.
Main Methods:
- Overexpression and knockdown studies of Ginir and Giniras in mouse cells.
- Co-immunoprecipitation assays to study protein-protein interactions.
- Analysis of mitotic regulation and genomic stability.
- Assessment of cellular proliferation and malignant transformation.
Main Results:
- Ginir acts as an oncogene, promoting cellular proliferation and malignant transformation.
- Ginir's oncogenic function is mediated by its interaction with centrosomal protein 112 (Cep112).
- Ginir disrupts the interaction between Cep112 and breast cancer type 1 susceptibility protein (Brca1), leading to mitotic abnormalities and genomic instability.
- Inhibition of Ginir in transformed cells attenuates malignant transformation and restores genomic stability.
Conclusions:
- Ginir is a novel lncRNA that plays a significant role in oncogenesis.
- The interaction between Cep112 and Brca1 is critical for maintaining mitotic fidelity and genomic stability.
- Ginir modulates the Cep112-Brca1 interaction, providing a new mechanism for lncRNA-mediated cancer development.
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