Related Experiment Video
Updated: Feb 4, 2026
09:05
Assessing Working Memory in Children: The Comprehensive Assessment Battery for Children – Working Memory (CABC-WM)
Published on: June 12, 2017
30.8K
Comprehensive assessment of biotin interference in immunoassays
Jieli Li1, Elizabeth A Wagar1, Qing H Meng1
1Department of Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Summary
Biotin, a common supplement, can interfere with immunoassay tests, causing inaccurate clinical results. Be cautious when interpreting abnormal lab values in patients taking biotin supplements.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Laboratory Medicine
Background:
- Biotinylated antibodies are common in immunoassays.
- Biotin is a popular dietary supplement.
- Biotin interference poses a growing challenge for clinical labs.
Purpose of the Study:
- To investigate the impact of biotin on immunoassay results.
- To identify specific analytes affected by biotin interference.
Main Methods:
- Prepared varying biotin concentrations in pooled patient serum.
- Analyzed analytes using sandwich and competitive immunoassays on the Roche Cobas 8000 e602 platform.
Main Results:
- Sandwich immunoassays showed falsely decreased results for some analytes.
- Competitive immunoassays showed falsely increased results for some analytes.
- Significant biotin interference observed in high-sensitivity troponin T, thyroid-stimulating hormone, follicle-stimulating hormone, triiodothyronine, and vitamin D assays.
Conclusions:
- Biotin significantly interferes with various immunoassays.
- Aberrant test results can occur in patients ingesting biotin.
- Clinicians must exercise caution when interpreting abnormal immunoassay results in biotin-supplemented patients.
Related Concept Videos
02:18Interference and Diffraction
52.4K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
52.4K
01:23RNA Interference
28.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.1K
01:16Interference and Decay
469
Forgetting is a complex cognitive phenomenon influenced by several factors, among which interference and decay are particularly prominent. These processes explain why individuals often struggle to retrieve specific information from memory, leading to lapses in recall that can be observed in everyday situations.
Interference occurs when competing memories hinder the retrieval of particular information. It can be classified into two types: proactive and retroactive interference. Proactive...
Interference occurs when competing memories hinder the retrieval of particular information. It can be classified into two types: proactive and retroactive interference. Proactive...
469
00:53Sound Waves: Interference
4.7K
Sound waves can be modeled either as longitudinal waves, wherein the molecules of the medium oscillate around an equilibrium position, or as pressure waves. When two identical waves from the same source superimpose on each other, the combination of two crests or two troughs results in amplitude reinforcement known as constructive interference. If two identical waves, that are initially in phase, become out of phase because of different path lengths, the combination of crests with troughs...
4.7K
01:07Interference and Superposition of Waves
6.9K
When two waves of the same nature occur in the same region simultaneously, they result in interference. Interference of waves implies that the net effect of the waves is the sum of the individual waves' effects. However, it does not imply that the individual waves affect the propagation of other waves.
Interference occurs in mechanical waves, such as sound waves, waves on a string, and surface water waves. Mechanical waves correspond to the physical displacement of particles. Hence,...
Interference occurs in mechanical waves, such as sound waves, waves on a string, and surface water waves. Mechanical waves correspond to the physical displacement of particles. Hence,...
6.9K
01:10Interference: Path Lengths
2.2K
Consider two sources of sound, that may or may not be in phase, emitting waves at a single frequency, and consider the frequencies to be the same.
Two special sources may be considered when they are in phase. This can be easily achieved by feeding the two sources from the same source. An example would be synchronizing the two speakers by feeding them with the same source, such as the sound waves produced by a tuning fork. This setup ensures that the two sources have the same frequency and are...
Two special sources may be considered when they are in phase. This can be easily achieved by feeding the two sources from the same source. An example would be synchronizing the two speakers by feeding them with the same source, such as the sound waves produced by a tuning fork. This setup ensures that the two sources have the same frequency and are...
2.2K