Micro-Raman high-pressure investigation on the malaria pigment hematin anhydride (β-hematin)

The effect of pressure on the Raman and fluorescence spectra of hematin anhydride (β-hematin) is reported. In a diamond-anvil cell, DAC, with applied pressures up to 41 kbar, the Raman spectrum undergoes a series of intensity enhancements and increases in energy for many of the Raman-active bands up to a pressure of ~27 kbar. At higher pressures, there is either a leveling out or a decrease in the energies of these vibrational modes. The fluorescence bands also undergo a series of pressure- sensitive changes where, up to 10 kbar, there is a marked quenching of the intensity of the emissive bands, which is accompanied by a net increase in energy of the vibrational bands. The results are interpreted in terms of a high-pressure phase change, to account for the Raman shifts, and a separate defect or surface site of the emissive state, which is more efficiently quenched at higher pressure.