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Live Cell Imaging during Mechanical Stretch
Published on: August 19, 2015
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Cyclic stretch increases mitochondrial biogenesis in a cardiac cell line
Hyoung Kyu Kim1, Yun Gyeong Kang2, Seung Hun Jeong3
1Cardiovascular and Metabolic Disease Center, Department of Physiology, Department of Health Sciences and Technology, BK21 Plus Project Team, College of Medicine, Bokji-ro 75, Busanjin-gu, Busan, 47392, South Korea; Department of Integrated Biomedical Science, College of Medicine, Inje University, Busanjin-gu, Busan, 47392, South Korea.
Biochemical and Biophysical Research Communications
|October 10, 2018
Summary
Cyclic stretch enhances cardiac cell mitochondria function by boosting gene and protein expression for biogenesis and oxidative phosphorylation. This improves mitochondrial mass and ATP production without harming cells.
Area of Science:
- Cardiovascular Biology
- Mitochondrial Physiology
- Cellular Biomechanics
Background:
- The heart relies on mitochondria for energy production to sustain continuous contraction and relaxation.
- Understanding how mechanical forces affect cardiac cell mitochondria is crucial for comprehending heart function and disease.
Purpose of the Study:
- To investigate the impact of heart mimetic cyclic stretch on mitochondrial biogenesis and oxidative phosphorylation in HL-1 murine cardiomyocytes.
- To determine if cyclic stretch affects cell viability and energy production in a cardiac cell line.
Main Methods:
- HL-1 cardiomyocytes were subjected to cyclic stretching (10% elongation, 0.5 Hz) on elastic micropatterned substrates.
- Gene expression (qPCR) and protein levels (Western blot) related to mitochondrial function were analyzed.
- Cell viability, mitochondrial mass, and ATP production were quantified.
Main Results:
- Cyclic stretch significantly upregulated genes involved in mitochondrial biogenesis (e.g., TFAM, PGC1-α) and oxidative phosphorylation (e.g., PHB1, CYTB).
- Protein levels of key mitochondrial biogenesis (TFAM, ERRα) and oxidative phosphorylation (NDUFS1, UQCRC, PHB1) factors were elevated.
- Treated cells exhibited increased mitochondrial mass and ATP production, with no adverse effects on cell viability.
Conclusions:
- Cyclic stretch effectively enhances mitochondrial biogenesis and oxidative phosphorylation at the transcriptional and translational levels in cultured cardiac cells.
- Mechanical stimulation via cyclic stretch represents a potential strategy to improve cardiac cell energy metabolism without compromising viability.
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