Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endurance training does not affect diaphragm mitochondrial respiration.

R F Fregosi, M Sanjak, D J Paulson

    Respiration Physiology
    |February 1, 1987
    PubMed
    Summary

    Chronic endurance training enhanced whole-body exercise capacity but did not increase mitochondrial respiration or protein content in rat diaphragm muscle. Plantaris muscle showed increased mitochondrial protein, suggesting differential adaptation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diverse physiological properties of hypoglossal motoneurons innervating intrinsic and extrinsic tongue muscles.

    Journal of neurophysiology·2019
    Same author

    Phrenicotomy alters phrenic long-term facilitation following intermittent hypoxia in anesthetized rats.

    Journal of applied physiology (Bethesda, Md. : 1985)·2010
    Same author

    Control of breathing in children with mild sleep apnoea: a 6-year follow-up study.

    The European respiratory journal·2009
    Same author

    Pharyngeal critical pressure in children with mild sleep-disordered breathing.

    Journal of applied physiology (Bethesda, Md. : 1985)·2006
    Same author

    Dissociation between mechanical and myoelectrical manifestation of muscle fatigue in amyotrophic lateral sclerosis.

    Amyotrophic lateral sclerosis and other motor neuron disorders : official publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases·2004
    Same author

    Coordination of intrinsic and extrinsic tongue muscles during spontaneous breathing in the rat.

    Journal of applied physiology (Bethesda, Md. : 1985)·2003

    Area of Science:

    • Exercise Physiology
    • Mitochondrial Biology
    • Skeletal Muscle Adaptation

    Background:

    • Chronic endurance training is known to induce adaptations in skeletal muscle.
    • The diaphragm muscle, crucial for respiration, may respond differently to training compared to limb muscles.
    • Understanding mitochondrial adaptations is key to comprehending exercise capacity improvements.

    Purpose of the Study:

    • To investigate the effects of chronic endurance training on mitochondrial respiration and protein content in rat diaphragm muscle.
    • To compare adaptations in the diaphragm with those in the plantaris muscle, a hindlimb muscle.
    • To determine if training enhances mitochondrial function or increases mitochondrial biogenesis in the diaphragm.

    Main Methods:

    • Twenty male Wistar rats were divided into control and 8-week endurance training groups.

    Related Experiment Videos

  • Cardiorespiratory fitness was assessed via VO2 max and peak power output.
  • Mitochondrial specific activities, oxygen uptake, and protein content were measured in diaphragm and plantaris muscles.

    • Main Results:

    • Endurance training significantly increased VO2 max and peak power output.
    • Plantaris muscle showed increased whole-muscle homogenate oxygen uptake and cytochrome oxidase activity, with a 50% increase in mitochondrial protein content.
    • Diaphragm muscle exhibited no significant changes in mitochondrial respiration or protein content, despite having higher baseline oxidative capacity than plantaris.

    Conclusions:

    • Endurance training enhances oxidative capacity in plantaris muscle by increasing mitochondrial protein content, not by improving existing mitochondrial function.
    • The diaphragm muscle's mitochondrial content and function do not adapt to this training stimulus, possibly due to its high baseline activity ('pre-adaptation').
    • The training intensity or duration may have been insufficient to induce significant mitochondrial adaptations in the diaphragm.