Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.0K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.0K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.6K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.6K
The Fluid Mosaic Model01:34

The Fluid Mosaic Model

178.1K
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
178.1K
Interference and Diffraction02:18

Interference and Diffraction

52.4K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
52.4K
CRISPR01:59

CRISPR

57.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.9K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.4K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of Polyacrylamide and Silicone Extracellular Matrix Substrates with Defined Stiffness for Cell Biology Applications.

Journal of visualized experiments : JoVE·2026
Same author

Tissue tension permits β-catenin phosphorylation to drive mesoderm specification in human embryonic stem cells.

Developmental cell·2026
Same author

NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer.

Nature communications·2026
Same author

Tissue tension fosters macrophage-driven lipid peroxidation-induced DNA damage.

Cancer cell·2026
Same author

Leveraging human genetic variation to therapeutically target hundreds of genes with dominant & dispensable disease alleles.

medRxiv : the preprint server for health sciences·2026
Same author

The Interplay Between Circadian Clocks and the Tumour Microenvironment in Breast Cancer.

Cancers·2026

Related Experiment Video

Updated: Feb 4, 2026

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

28.1K

Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference.

Ashley Rg Libby1,2, David A Joy1,3, Po-Lin So1

  • 1Gladstone Institute of Cardiovascular Disease, San Francisco, United States.

Elife
|October 10, 2018
PubMed
Summary

Researchers developed a novel mosaic patterning method in human induced pluripotent stem cells (hiPSCs) to study cell interactions during morphogenesis. This technique allows precise genetic manipulation of specific cell subpopulations, revealing insights into self-organization and symmetry-breaking.

Keywords:
bio-engineeringdevelopmental biologyhumanmorphogenesispluripotent stem cellsregenerative medicinestem cells

More Related Videos

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.8K
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

1.3K

Related Experiment Videos

Last Updated: Feb 4, 2026

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

28.1K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.8K
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

1.3K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • Morphogenesis relies on complex cell-cell interactions and differentiation.
  • Current models lack precise control over cell subpopulation behavior and perturbation.
  • Understanding symmetry-breaking in multicellular development is crucial.

Purpose of the Study:

  • To develop an in vitro system for studying cell-cell interactions and multicellular organization in human induced pluripotent stem cell (hiPSC) colonies.
  • To investigate the effects of modulating cortical tension and cell-cell adhesion on hiPSC colony patterning.
  • To enable genetic interrogation of emergent multicellular properties and pathways regulating morphogenesis.

Main Methods:

  • Utilized CRISPR interference for induced mosaic knockdown of ROCK1 (cortical tension) and CDH1 (cell-cell adhesion) in hiPSC colonies.
  • Observed cellular self-organization and differential patterning within the mosaic hiPSC colonies.
  • Analyzed gene expression changes in response to knockdown and coordinated with self-organization.

Main Results:

  • Mosaic knockdown of ROCK1 or CDH1 induced differential patterning within hiPSC colonies via cellular self-organization.
  • The pluripotent epithelial phenotype was maintained despite genetic perturbations.
  • A transient wave of differential gene expression was observed, stabilizing with self-organization.

Conclusions:

  • Mosaic patterning in hiPSC colonies provides a powerful tool for genetic interrogation of emergent multicellular properties.
  • This system facilitates a deeper understanding of the molecular pathways governing symmetry-breaking during morphogenesis.
  • The method allows selective perturbation of cell subpopulations to study their role in tissue development.