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Updated: Feb 4, 2026

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
Published on: June 12, 2021
Distribution of tumor-infiltrating immune cells in glioblastoma
Enrique Orrego1, Carlos A Castaneda2,3, Miluska Castillo2
1Neurosurgery Department, Instituto Nacional de Enfermedades Neoplasicas, Lima, 15038, Peru.
Aim:
Evaluation of features related to infiltrating immune cell level in glioblastoma.
Methods:
Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis.
Results:
CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival.
Conclusion:
Macrophages are more frequent than TILs. Some subsets are associated with clinical features.
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