Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation01:22

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation

932
Glutathione, a tripeptide made up of glutamate, cysteine, and glycine, is a critical player in the detoxification of drugs and xenobiotics via a process known as glutathione conjugation or mercapturic acid formation. This phase II biotransformation reaction involves the covalent binding of glutathione to a drug or its metabolite, enhancing the compound's water solubility and enabling its excretion.
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
932
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.3K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.3K
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

1.3K
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
1.3K
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

1.1K
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
1.1K
Local Anesthetics: Differential Sensitivity of Nerve Fibers01:24

Local Anesthetics: Differential Sensitivity of Nerve Fibers

1.4K
Local anesthetics (LAs) block the sodium channels of nerve trunks, sensory nerve endings, and neuromuscular junctions. Although LAs can block all kinds of nerves, the sensitivity of nerve fibers differs according to nerve types and structures. LAs are known to block myelinated fibers faster than unmyelinated ones. Also, they block pain or sensory neurons at low concentrations without affecting the motor neurons involved in muscle contractions. This helps relieve labor pain without affecting the...
1.4K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

24.3K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
24.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibitors of the Machupo Virus L Endonuclease for Bolivian Hemorrhagic Fever Treatments.

Microorganisms·2026
Same author

Vagus nerve-dependent antidepressant effects of ineupatorolide B via gut microbiota modulation in a mouse model of depression.

Translational psychiatry·2026
Same author

Huaganjian decoction attenuates liver fibrosis via purine metabolic-inflammatory synergistic crosstalk: network pharmacology combined with multi-omics analyses.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Covalent tumor anchoring spatially orchestrates antitumor immunity.

bioRxiv : the preprint server for biology·2026
Same author

Linker Nitrogen Tunes Charge Polarity to Strengthen Built-In Electric Fields in Covalent Organic Frameworks for Photocatalytic Oxidation.

Angewandte Chemie (International ed. in English)·2026
Same author

Oral Colon-Targeted Lipid Nanoparticles Enhance Upadacitinib Delivery and Efficacy in a Murine Model of Ulcerative Colitis.

International journal of molecular sciences·2026
Same journal

Y(OTf)<sub>3</sub>-Catalyzed Formal (3 + 2) Cycloadditions of Donor-Acceptor Cyclopropanes with KSCN under Mechanochemical Conditions.

Organic letters·2026
Same journal

Electro-Mediated Regioselective C2-Alkylation of <i>N</i>-Oxide Heteroarenes.

Organic letters·2026
Same journal

Organophosphine-Promoted Decarbynylative Hydrocarbenylation of the Carbon-Carbon Triple Bond.

Organic letters·2026
Same journal

Total Syntheses of BE-54238A and -B.

Organic letters·2026
Same journal

Visible Light-Induced <i>N</i>-Phenylbenzo[<i>c</i>]phenothiazine-Catalyzed α-C(sp<sup>3</sup>)-H Phosphonylation of Secondary Amines via Intramolecular 1,5-HAT.

Organic letters·2026
Same journal

Cobalt-Stabilized Propargylic Oxocarbenium Ions Enable Direct and Asymmetric Nickel(II) Catalyzed Aldol-Like Reactions.

Organic letters·2026
See all related articles

Related Experiment Video

Updated: Feb 4, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

19.6K

Esterase-Sensitive Glutathione Persulfide Donor.

Zhengnan Yuan1, Yueqin Zheng1, Bingchen Yu1

  • 1Department of Chemistry and Center for Diagnostics and Therapeutics , Georgia State University , Atlanta , Georgia 30303 , United States.

Organic Letters
|October 10, 2018
PubMed
Summary
This summary is machine-generated.

A novel glutathione persulfide (GSSH) donor, BW-GP-401, was developed. This compound demonstrates GSSH release and offers cytoprotective effects in cardiomyocytes under oxidative stress.

More Related Videos

Measuring Glutathione-induced Feeding Response in Hydra
08:10

Measuring Glutathione-induced Feeding Response in Hydra

Published on: November 16, 2014

11.7K
Single Port Donor Nephrectomy
07:17

Single Port Donor Nephrectomy

Published on: March 12, 2011

52.4K

Related Experiment Videos

Last Updated: Feb 4, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

19.6K
Measuring Glutathione-induced Feeding Response in Hydra
08:10

Measuring Glutathione-induced Feeding Response in Hydra

Published on: November 16, 2014

11.7K
Single Port Donor Nephrectomy
07:17

Single Port Donor Nephrectomy

Published on: March 12, 2011

52.4K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Medicinal Chemistry

Background:

  • Glutathione persulfide (GSSH) plays a crucial role in cellular redox homeostasis.
  • Dysregulation of GSSH levels is implicated in various oxidative stress-related pathologies.
  • Development of effective GSSH donors is essential for therapeutic interventions.

Purpose of the Study:

  • To synthesize and characterize a novel esterase-sensitive glutathione persulfide (GSSH) donor, designated BW-GP-401.
  • To investigate the release kinetics and mechanism of BW-GP-401.
  • To evaluate the biological activity of BW-GP-401, including its inhibitory effects on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and its cytoprotective properties.

Main Methods:

  • Synthesis of BW-GP-401, an esterase-sensitive GSSH donor.
  • Monitoring GSSH release by measuring lactone formation and using 1-fluoro-2,4-dinitrobenzene (DNFB) for direct trapping.
  • Assessing the inhibitory effect of BW-GP-401 on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) activity.
  • Evaluating cytoprotective effects in H9c2 cardiomyocytes under oxidative conditions.

Main Results:

  • Successful synthesis and characterization of the GSSH donor BW-GP-401.
  • Demonstrated esterase-dependent release of GSSH from BW-GP-401.
  • BW-GP-401 exhibited inhibitory effects on GAPDH activity.
  • Significant cytoprotective effects of BW-GP-401 were observed in H9c2 cardiomyocytes exposed to oxidative stress.

Conclusions:

  • BW-GP-401 serves as an effective esterase-sensitive donor for glutathione persulfide (GSSH).
  • The compound modulates GAPDH activity and provides cytoprotection against oxidative damage in cardiomyocytes.
  • BW-GP-401 holds potential for therapeutic strategies targeting redox imbalance and oxidative stress.