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Related Concept Videos

Interaction of EM Radiation with Matter: Spectroscopy01:12

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Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in its path. The electric field in the radiation can interact with electrical charges in the atoms or molecules in the matter. On the other hand, the magnetic field can interact with the magnetic field in the atomic nucleus. The study of the interaction between electromagnetic radiation and matter is termed spectroscopy. Spectroscopy is the study...
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Electromagnetic (EM) radiation consists of electric and magnetic field components oscillating in planes perpendicular to each other and mutually perpendicular to radiation propagation through space. EM radiation can be classified as a wave, characterized by the properties of waves such as wavelength (denoted as λ) and frequency (represented by ν).
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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Related Experiment Video

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Preparation of High-Temperature Sample Grids for Cryo-EM
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Cryo-EM.

Eva Nogales1

  • 1QB3 Institute, UC Berkeley, Molecular and Cell Biology Department, UC Berkeley, Howard Hughes Medical Institute, Molecular Biophysics and Integrative Bioimaging Division, Lawrence Berkeley National Lab, Stanley Hall, UC Berkeley, Berkeley, CA 94720-3220, USA.

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|October 10, 2018
PubMed
Summary
This summary is machine-generated.

Cryo-electron microscopy provides unprecedented views of cellular machinery. This technique allows scientists to see complex biological molecules in high detail.

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Area of Science:

  • Structural biology
  • Biophysics

Background:

  • Understanding macromolecular structures is crucial for deciphering biological functions.
  • Traditional methods for visualizing these structures have limitations.

Purpose of the Study:

  • To introduce and explain the principles of cryo-electron microscopy (cryo-EM).
  • To highlight the capabilities of cryo-EM in visualizing macromolecular structures.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) is presented as a cutting-edge imaging technique.
  • The process involves flash-freezing samples and imaging them with an electron microscope.

Main Results:

  • Cryo-EM enables the visualization of biological molecules at near-atomic resolution.
  • This technique reveals the intricate three-dimensional arrangements of proteins and other macromolecules.

Conclusions:

  • Cryo-electron microscopy is a powerful tool revolutionizing structural biology.
  • It offers new avenues for understanding cellular mechanisms and disease pathways.