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Updated: Feb 4, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using
Yoshiki Morifuji1, Jian Xu2, Noriko Karasaki1
1Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Science, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan.
Abstract:
Human α1-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α1-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). In this study, the baculovirus expression vector system using silkworm larvae as host was employed and a large amount of highly active AAT was recovered from the silkworm serum (~ 15 mg/10 ml) with high purity. Both the enzymatic activity and stability of purified rAAT were comparable with those of commercial product. Our results provide an alternative method for mass production of the active rAAT in pharmaceutical use.
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