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Updated: Feb 4, 2026

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
Published on: August 6, 2025
Streptomyces durbertensis sp. nov., isolated from saline-alkali soil
Yang Yu1, Yansong Fu1, Xiaowei Guo1
11Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
Abstract:
A novel strain of actinobacteria, designated NEAU-S1GS20T, was isolated from a saline-alkali soil collected from Heilongjiang Province, north-east China, and characterized using a polyphasic approach. Strain NEAU-S1GS20T exhibited morphological, cultural and chemotaxonomic features consistent with its classification as representing a member of the genus Streptomyces. Growth occurred at 18‒45 °C, at pH 6.0‒10.0 and in the presence of 10 % (w/v) NaCl. Whole-cell hydrolysates mainly contained glucose and ribose. The predominant menaquinones were MK-9(H4) and MK-9(H6). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unidentified phospholipids. The major cellular fatty acids (>10 %) were iso-C16 : 0, iso-C17 : 0, anteiso-C17 : 0 and C16 : 0. The G+C content of the DNA was 72.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain NEAU-S1GS20T formed a distinct clade within the genus Streptomyces and was closely related to Streptomyces xinghaiensis CCTCC AA 208049T (98.4 % similarity), Streptomyces chumphonensis JCM 18522T (98.1 %) and Streptomyces palmae JCM 31289T (98.1 %). Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA distance of strain NEAU-S1GS20T to the most closely related species was greater than the 0.007 threshold. A combination of DNA-DNA hybridization results and differences in certain phenotypic characteristics demonstrated that strain NEAU-S1GS20T could be distinguished from its closest phylogenetic relatives. Therefore, strain NEAU-S1GS20T represents a novel species of the genus Streptomyces, for which the name Streptomyces durbertensis sp. nov. is proposed. The type strain is NEAU-S1GS20T (=CCTCC AA 2017006T=DSM 104538T).
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