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Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
Natália Marcondes1,2, Flavo Fernandes2, Gustavo Faulhaber1,2,3
1Programa de Pós Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Revista Da Associacao Medica Brasileira (1992)
|October 11, 2018
Summary
Flow cytometry can assess Ki-67 expression, a marker of cell proliferation, in mature B-cell neoplasms. This method shows higher Ki-67 levels in aggressive lymphomas, aiding diagnosis.
Area of Science:
- Hematology
- Oncology
- Immunology
Background:
- Ki-67 is a nuclear protein indicating cellular proliferation.
- Immunohistochemistry is the traditional method for Ki-67 evaluation.
- Mature B-cell neoplasms can exhibit varying degrees of aggressiveness.
Purpose of the Study:
- To evaluate Ki-67 expression in mature B-cell neoplasms using flow cytometry immunophenotyping.
- To determine the utility of flow cytometry for Ki-67 assessment in these conditions.
Main Methods:
- Mature B-cell samples underwent surface staining for CD19 and CD45.
- Intracellular staining for Ki-67 was performed on leukemic mature B-cells.
- Ki-67 expression was quantified using flow cytometry.
Main Results:
- Elevated Ki-67 expression was observed in mantle cell lymphoma, Burkitt lymphoma, and diffuse large B-cell lymphoma.
- Ki-67 levels correlated with CD38 mean fluorescence intensity.
Conclusions:
- Flow cytometry is a viable method for assessing Ki-67 expression in mature B-cell neoplasms.
- Further research is necessary to validate Ki-67 assessment by flow cytometry immunophenotyping.
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