Metabotropic glutamate receptor 1-mediated calcium mobilization in the neonatal hippocampal marginal zone

The hippocampal marginal zone contains Cajal-Retzius (C-R) cells and participates in the regulation of cortical development. Two subtypes of group I metabotropic glutamate receptors (mGluRs), mGluR1 and mGluR5, are found in the central nervous system and are considered to regulate neuronal excitability. The release of Ca²⁺ from intracellular stores is thought to be a main consequence of activation of these receptor subtypes. In hippocampal C-R cells, the expression of mGluR1 has been showed using immunohistochemical techniques, but its function has not been elucidated. In this study, Ca²⁺ mobilization through mGluR1 activation was demonstrated in the neonatal rat hippocampus. In marginal zone C-R cells, intracellular Ca²⁺ elevation was detected by fluorescence imaging after the application of a group I mGluR-specific agonist. This response was prevented by application of an mGluR1 antagonist but was not changed by application of an mGluR5 antagonist. The intracellular Ca²⁺ elevation induced by mGluR1 activation was still observed in Ca²⁺ -free perfusate, indicating the release of Ca²⁺ from intracellular stores. γ-Aminobutyric acid and ionotropic glutamate receptor-mediated intracellular Ca²⁺ elevation was also detected in mGluR1-possessing neurons, although the former was much smaller than that mediated by mGluR1. These results indicate that mGluR1 is functionally expressed in C-R cells in the neonatal marginal zone and regulates cell function through the elevation of intracellular Ca²⁺ .