Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pole and System Stability01:24

Pole and System Stability

966
The transfer function is a fundamental concept representing the ratio of two polynomials. The numerator and denominator encapsulate the system's dynamics. The zeros and poles of this transfer function are critical in determining the system's behavior and stability.
Simple poles are unique roots of the denominator polynomial. Each simple pole corresponds to a distinct solution to the system's characteristic equation, typically resulting in exponential decay terms in the system's...
966
Angle of Twist: Problem Solving01:13

Angle of Twist: Problem Solving

794
An electric motor applies a torque of 700 N·m to an aluminum shaft, triggering a stable rotation. Two pulleys, B and C, are subjected to torques of 300 N·m and 400 N·m, respectively. The modulus of rigidity is provided as 25 GPa. With the knowledge of the length and diameter of each segment, the twist angle between the two pulleys can be computed. First, a section cut is made between pulleys B and C, and the cut cross-section is analyzed using a free-body diagram. Given that the torque...
794
Angle of Twist - Elastic Range01:13

Angle of Twist - Elastic Range

808
Consider a cylindrical shaft with a length denoted by L and a consistent cross-sectional radius referred to as r. This shaft undergoes a torque at the free end. The highest shearing strain within the shaft is directly proportional to the twist angle and the radial distance from the shaft axis. When the shaft behaves elastically, this shearing strain can be articulated using variables such as the applied torque, radial distance, the polar moment of inertia, and the modulus of rigidity. By...
808
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.1K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.1K
What are Membranes?01:54

What are Membranes?

190.7K
A key characteristic of life is the ability to separate the external environment from the internal space. To do this, cells have evolved semi-permeable membranes that regulate the passage of biological molecules. Additionally, the cell membrane defines a cell’s shape and interactions with the external environment. Eukaryotic cell membranes also serve to compartmentalize the internal space into organelles, including the endomembrane structures of the nucleus, endoplasmic reticulum and...
190.7K
What are Membranes?01:24

What are Membranes?

19.0K
A cell's plasma membrane demarcates the cell's borders and determines the nature of its interaction with the environment. Cells exclude certain substances, take in others, and excrete some others in controlled quantities. The plasma membrane must be flexible to allow certain cells, such as red and white blood cells, to change their shape while passing through narrow capillaries. These are the more obvious plasma membrane functions. In addition, the plasma membrane's surface carries...
19.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

F-actin flashes on phagosomes mechanically deform contents for efficient digestion in macrophages.

Journal of cell science·2020
Same author

Binding and uptake of single and dual-opsonized targets by macrophages.

Journal of cellular biochemistry·2019
See all related articles

Related Experiment Video

Updated: Feb 4, 2026

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
08:05

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Published on: May 27, 2021

3.2K

A tent pole twist on membrane ruffles.

Reuben V Philip1, Rene E Harrison1

  • 1University of Toronto, Toronto, Canada.

The Journal of Cell Biology
|October 12, 2018
PubMed
Summary

Researchers discovered "tent poles," parallel membrane structures that guide the formation of membrane ruffles during macropinocytosis, or cell drinking. This finding offers new insights into cellular fluid uptake mechanisms.

Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Macropinocytosis is a cellular process for internalizing extracellular fluids.
  • It involves the formation of dynamic membrane structures called ruffles.

Purpose of the Study:

  • To investigate the structural mechanisms guiding membrane ruffle formation during macropinocytosis.

Main Methods:

  • Utilized lattice light sheet microscopy for high-resolution live-cell imaging.
  • Observed cellular dynamics during macropinocytosis.

Main Results:

  • Identified novel parallel membrane protrusions, termed 'tent poles'.
  • Demonstrated that these 'tent poles' flank and direct the formation of membrane ruffles.
  • Provided a new structural model for macropinocytosis initiation.

More Related Videos

Magnetic Tweezers for the Measurement of Twist and Torque
11:41

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

23.9K
High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal
06:24

High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal

Published on: October 31, 2019

6.9K

Related Experiment Videos

Last Updated: Feb 4, 2026

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
08:05

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Published on: May 27, 2021

3.2K
Magnetic Tweezers for the Measurement of Twist and Torque
11:41

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

23.9K
High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal
06:24

High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal

Published on: October 31, 2019

6.9K

Conclusions:

  • 'Tent poles' play a crucial role in orchestrating macropinocytosis.
  • This discovery enhances our understanding of cellular fluid uptake and membrane dynamics.