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Updated: Feb 4, 2026

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
11:50

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

Published on: September 10, 2014

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PRKRA/PACT Expression Promotes Chemoresistance of Mucinous Ovarian Cancer

Takeshi Hisamatsu1, Michael McGuire1, Sherry Y Wu1

  • 1Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Molecular Cancer Therapeutics
|October 12, 2018
PubMed

Insights

Mucinous ovarian cancer (MOC) is resistant to standard chemotherapy. Researchers identified the PRKRA/PACT pathway and miR-515-3p as key targets to enhance oxaliplatin efficacy in MOC models.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Mucinous ovarian cancer (MOC) exhibits resistance to conventional platinum-based chemotherapy.
  • Understanding mechanisms of oxaliplatin resistance is crucial for developing effective MOC treatments.

Purpose of the Study:

  • To identify novel therapeutic targets for overcoming oxaliplatin resistance in MOC.
  • To investigate the role of the PRKRA/PACT axis and miR-515-3p in MOC chemosensitivity.

Main Methods:

  • A kinome-based siRNA library screen was employed using human MOC cells.
  • In vitro and in vivo studies utilized mouse MOC models for validation.
  • The PRKRA/PACT pathway and miR-515-3p regulation of AXL were investigated.

Main Results:

  • The PRKRA/PACT axis was identified as a significant factor in oxaliplatin resistance.
  • siRNA targeting PRKRA combined with oxaliplatin demonstrated significant antitumor effects in vivo.
  • miR-515-3p, regulated by PACT-Dicer interaction, enhanced MOC sensitivity to oxaliplatin by targeting AXL.

Conclusions:

  • The PRKRA/PACT axis is a critical therapeutic target for enhancing oxaliplatin sensitivity in MOC.
  • Modulating miR-515-3p offers a potential strategy to improve MOC treatment outcomes.

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