Surface Functionalized Prussian Blue-coated Nanostructured Nickel Oxide as a New Biosensor Platform for Catechol...

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biotechnology

Background:

• Catechol detection is crucial for environmental and biological monitoring.
• Developing sensitive and selective biosensors is an ongoing challenge.
• Nanomaterials offer unique properties for biosensor development.

Purpose of the Study:

• To develop a highly efficient and sensitive amperometric biosensor for catechol detection.
• To utilize Prussian blue-coated nickel oxide nanoparticles as a matrix for tyrosinase immobilization.
• To investigate the electrochemical performance of the developed biosensor.

Main Methods:

• Synthesis of Prussian blue (PB)-coated nickel oxide (NiO) nanoparticles (NPs) via sol-gel method.
• Immobilization of tyrosinase enzyme onto PB-NiO NPs deposited on a screen-printed carbon electrode (SPCE).
• Electrochemical characterization using cyclic voltammetry (CV) and chronoamperometry.

Main Results:

• Successful synthesis and characterization of PB-NiO NPs using TEM and EDS.
• Verification of enzyme immobilization using AFM and SEM.
• Achieved high sensitivity (0.954 μA/μM), wide linear range (1-50 μM), and low LOD (0.087 μM) for catechol.
• Demonstrated fast response time (27 s) and good selectivity.

Conclusions:

• The developed PB-NiO/tyrosinase biosensor provides a sensitive, selective, and efficient platform for catechol detection.
• The use of PB-coated NiO NPs enhances electrocatalytic activity and biosensor performance.
• This biosensor holds potential for practical applications in monitoring catechol levels.