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Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
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MiR-200b attenuates IL-6 production through IKKβ and ZEB1 in human gingival fibroblasts

Sari Matsui1, Liming Zhou1,2, Yohei Nakayama1,3

  • 1Department of Periodontology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, 271-8587, Japan.

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|October 12, 2018
PubMed
Abstract

Insights

MicroRNA 200b (miR-200b) reduces inflammation in periodontal tissue by suppressing interleukin-6 (IL-6) and targeting key inflammatory regulators. This microRNA plays a protective role in the inflamed gingiva.

Area of Science:

  • Molecular Biology
  • Immunology
  • Periodontology

Background:

  • MicroRNAs (miRNAs) regulate critical biological processes, including inflammation and tumorigenesis.
  • Previous studies indicated elevated miR-200b in inflamed gingiva.
  • The specific role of miR-200b in gingival inflammation requires elucidation.

Purpose of the Study:

  • To investigate the functional role of miR-200b in human gingival fibroblasts (HGF).
  • To analyze the effect of miR-200b on interleukin-6 (IL-6) expression in HGF.
  • To explore the molecular mechanisms underlying miR-200b's action in inflamed periodontal tissues.

Main Methods:

  • Human gingival fibroblasts (HGF) were stimulated with IL-1β or TNF-α.
  • Cells were transfected with miR-200b expression plasmid or inhibitor.
  • mRNA and protein levels of IL-6, IKKβ, ZEB1, and E-cadherin were quantified using real-time PCR and Western blot.

Main Results:

  • IL-1β and TNF-α significantly increased IL-6 expression, which was suppressed by miR-200b overexpression.
  • miR-200b inhibitor further elevated IL-6 levels.
  • miR-200b suppressed inhibitor of nuclear factor kappa-B kinase beta (IKKβ) and Zinc-finger E-box-binding homeobox 1 (ZEB1) expression, while increasing E-cadherin levels.

Conclusions:

  • miR-200b attenuates inflammatory responses in periodontal tissues.
  • The mechanism involves the suppression of IKKβ and ZEB1.
  • miR-200b plays a protective role against inflammation in the gingiva.
Keywords:
Human gingival fibroblastsIKKβIL-6PeriodontitisZEB1miR-200b

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