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Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain
Qiaoli Ma1, Miriam Ries1, Yann Decker2
1Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Vladimir-Prelog-Weg 1-5/10, HCI H398, 8093, Zurich, Switzerland.
Acta Neuropathologica
|October 12, 2018
Summary
Cerebrospinal fluid (CSF) rapidly exits the brain via lymphatics in awake mice, preventing significant influx into brain tissue. Anesthesia alters tracer distribution, highlighting the importance of physiological conditions in studying CSF dynamics.
Area of Science:
- Neuroscience
- Physiology
- Fluid Dynamics
Background:
- The exchange between cerebrospinal fluid (CSF) and brain interstitial fluid is not fully understood.
- Proposed mechanisms include CSF flow through paravascular spaces and lymphatic drainage.
- The relative contribution of these pathways remains under investigation.
Purpose of the Study:
- To investigate the functional relationship between CSF lymphatic efflux and brain influx.
- To assess the distribution of CSF-infused tracers in awake versus anesthetized mice.
Main Methods:
- Near-infrared fluorescence imaging in awake mice.
- Magnetic resonance imaging and fluorescence microscopy in anesthetized mice.
- Assessment of tracer distribution in brain paravascular spaces and parenchyma.
Main Results:
- In awake mice, tracers rapidly entered the lymphatic system and systemic circulation from the subarachnoid space.
- Anesthetized mice showed tracers confined to superficial paravascular spaces.
- Significant tracer influx into the brain parenchyma occurred only after death.
Conclusions:
- Rapid CSF lymphatic drainage in awake animals limits bulk flow into the brain parenchyma.
- Anesthesia may alter CSF dynamics and tracer distribution.
- Lymphatic clearance is a primary route for CSF turnover under physiological conditions.
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