Protein methylase II in five taxa from three phyla

Protein methylase II (protein O-methyltransferase, EC 2.1.1.24) was found in Dictyostelium discoideum amoebae, Astacus leptodactylus axonal, Locusta migratoroides neuronal, Torpedo marmorata electroplaque and Bos bovis stratial tissue and compared in both the soluble and particulate fractions. The intrinsic decay data of the methyl groups transferred onto proteins from Dictyostelium and Torpedo tissues were virtually identical. The short term kinetics of the methyl group transfer of all fractions and of all taxa investigated were non-linear and multiphasic. The particulate fractions displayed transient peaks at 1 min, 3 min, or both after the start of the reaction. The methyl group transfer was stimulated by the neurotoxins veratridine (VTx) and inhibited by veratridine plus tetrodotoxin (TTx) (axonal membrane vesicles of Astacus), stimulated transiently and in a biphasic manner by carbamoylcholine and phospholipase A2 (AChR-rich membrane vesicles of Torpedo), and stimulated transiently and biphasically by the adequate chemotactic stimulus cAMP (aggregation competent amoebae of Dictyostelium).