Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Complex Ions03:45

Formation of Complex Ions

26.1K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.1K
Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

849
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
849
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

1.6K
Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
1.6K
Factors Influencing Drug Absorption: Drug Dissolution01:27

Factors Influencing Drug Absorption: Drug Dissolution

1.2K
The pharmacokinetic journey of drugs from solid oral dosage forms into systemic circulation is multifaceted. It begins with disintegration, a prerequisite ensuring a solid dosage form's subdivision into minute particles. Dissolution occurs next as these granulated entities solubilize in gastrointestinal fluids. This solubilization is crucial for the succeeding stage, permeation, which describes the traversal of the drug across the intestinal membrane and its subsequent entry into the blood...
1.2K
Formal Charges02:42

Formal Charges

40.5K
In some cases, there are seemingly more than one valid Lewis structures for molecules and polyatomic ions. The concept of formal charges can be used to help predict the most appropriate Lewis structure when more than one reasonable structure exists.
40.5K
Hepatic Drug Excretion: Influencing Factors01:16

Hepatic Drug Excretion: Influencing Factors

581
The biliary system of the liver, crucial for bile secretion and drug excretion, comprises intrahepatic bile ducts that merge to form the common hepatic duct. This duct, carrying hepatic bile, combines with the cystic duct, draining the gallbladder and forming the common bile duct, which empties into the duodenum. Bile, produced by hepatic cells lining the bile canaliculi, is composed primarily of water, bile salts, pigments, electrolytes, and lesser amounts of cholesterol and fatty acids. Bile...
581

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electricity generation from food wastes and microbial community structure in microbial fuel cells.

Bioresource technology·2013
Same author

Plasmon-enhanced photothermoelectric conversion in chemical vapor deposited graphene p-n junctions.

Journal of the American Chemical Society·2013
Same author

Rapid and sensitive detection of vinorelbine in the urine of tumor patients by capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II)-based electrochemiluminescence assay.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2013
Same author

Synthesis and biological evaluation of 4-phenoxy-6,7-disubstituted quinolines possessing semicarbazone scaffolds as selective c-Met inhibitors.

Archiv der Pharmazie·2013
Same author

The effects of external electric field: creating non-zero first hyperpolarizability for centrosymmetric benzene and strongly enhancing first hyperpolarizability for non-centrosymmetric edge-modified graphene ribbon H2N-(3,3)ZGNR-NO2.

Journal of molecular modeling·2013
Same author

Msx2 plays a critical role in lens epithelium cell cycle control.

International journal of ophthalmology·2013

Related Experiment Video

Updated: Feb 4, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.0K

Pullulan-Based Nanoparticle-HSA Complex Formation and Drug Release Influenced by Surface Charge.

Liming Yuan1, Yiting Cao1, Qian Luo1

  • 1Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Medicine, Hunan Normal University, Changsha, 410013, China.

Nanoscale Research Letters
|October 12, 2018
PubMed
Summary

Researchers explored how nanoparticle (NP) surface chemistry affects protein interactions and drug release. Modifying pullulan NPs with different charges influenced human serum albumin (HSA) binding and significantly reduced drug release rates, highlighting NP design importance.

Keywords:
Binding constantDrug releaseHuman serum albuminNanoparticlesα-Helix

More Related Videos

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

8.0K
Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.8K

Related Experiment Videos

Last Updated: Feb 4, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.0K
Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

8.0K
Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.8K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery

Background:

  • Nanoparticle (NP) design is crucial for drug delivery, with protein adsorption in blood significantly impacting efficacy.
  • Understanding nanoparticle-protein interactions is key to optimizing drug-loaded NP performance.
  • Pullulan-based NPs offer a versatile platform for surface modification to study these interactions.

Purpose of the Study:

  • To synthesize and characterize pullulan nanoparticles (NPs) with varying surface charges (positive, negative, neutral).
  • To investigate the interaction between these modified NPs and human serum albumin (HSA).
  • To evaluate the impact of NP-HSA complexation on drug release kinetics.

Main Methods:

  • Synthesis of cholesteric hydrophobically (CH) modified pullulan (CHP), CH-modified animated pullulan (CHAP), and CH-modified carboxylated pullulan (CHSP) nanoparticles.
  • Characterization of NP size and zeta potential using dynamic light scattering.
  • Thermodynamic analysis of NP-HSA interactions via isothermal titration calorimetry.
  • Assessment of protein structural changes using fluorescence and circular dichroism spectroscopy.
  • Measurement of drug release rates from NP-HSA complexes.

Main Results:

  • CHAP, CHSP, and CHP NPs exhibited distinct zeta potentials (+12.9, -15.4, -0.698 mV) and sizes (116.9, 156.9, 73.1 nm).
  • NP surface charge significantly influenced HSA binding affinity, with CHAP showing the highest binding constant (412 × 10^4 M⁻¹).
  • Complexation with NPs altered HSA structure, reducing alpha-helical content, with CHP causing the greatest reduction.
  • NP-HSA complexation significantly lowered drug release rates compared to free drug or drug-loaded NPs, with CHSP-HSA and CHP-HSA showing the highest and lowest rates, respectively.

Conclusions:

  • Nanoparticle surface charge and size are critical factors modulating protein adsorption and subsequent drug release.
  • The interaction between nanoparticles and HSA is complex, involving structural protein changes and affecting drug delivery profiles.
  • Tailoring nanoparticle surface chemistry is essential for controlling protein interactions and optimizing the performance of drug delivery systems.