Long noncoding RNA neuroblastoma-associated transcript 1 gene inhibits malignant cellular phenotypes of bladder
Zhongyuan Liu1, Dalong Xie2, Hui Zhang3
1Department of Urinary surgery, Shengjing Hospital, China Medical University, Shenyang, 110004, China.
Abstract:
Bladder cancer (BC) is one of the most common tumors in the urinary system. Noncoding RNAs are considered to take part in cellular phenotypes and are emerging as diagnostic and prognostic biomarkers of BC. The aim of this study is to investigate the clinical significance of neuroblastoma- associated transcript 1 (NBAT1) gene and its effects on malignant cellular phenotypes in BC. NBAT1 gene was low-expressed in BC tissues and cell lines and its low-expression was related with high pathological grade and metastasis of BC. Upregulation of NBAT1 gene depressed cell viability and invasiveness of KK47 and T24 cells and arrested KK47 and T24 cells at G1 stage. In addition, NBAT1 could target silence the expression of miR-21-5p in RNA-induced silencing complex-dependent manner. KK47 and T24 cells with miR-21-5p knockdown showed reduced cell viability, G1-stage arrest, and depressed invasiveness. MiR-21-5p mediates the regulatory effects of NBAT1 on malignant cellular phenotypes of BC cells. Moreover, SOCS6 gene was a target gene of miR-21-5p, and miR-21-5p modulated malignant cellular phenotypes of KK47 and T24 cells through targeted silencing of SOCS6. In conclusion, low-expression of NBAT1 is associated with the progress and metastasis of BC, and NBAT1 inhibits malignant cellular phenotypes through miR-21-5p/SOCS6 axis in BC. Our findings help to elucidate the tumorigenesis of BC, and future study will provide a novel therapeutic target for BC.
Insights
Low expression of neuroblastoma-associated transcript 1 (NBAT1) correlates with bladder cancer (BC) progression and metastasis. NBAT1 suppresses BC cell malignancy by regulating the miR-21-5p/SOCS6 axis, offering a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Bladder cancer (BC) is a prevalent urinary system malignancy.
- Noncoding RNAs are implicated in BC pathogenesis and serve as potential biomarkers.
- Neuroblastoma-associated transcript 1 (NBAT1) function in BC remains underexplored.
Purpose of the Study:
- To investigate the clinical significance of NBAT1 in BC.
- To elucidate the role of NBAT1 in BC cellular phenotypes.
- To identify the molecular mechanisms underlying NBAT1's function in BC.
Main Methods:
- Analysis of NBAT1 expression in BC tissues and cell lines.
- Functional assays (cell viability, invasion, cell cycle analysis) following NBAT1 or miR-21-5p manipulation.
- Investigation of the regulatory relationship between NBAT1, miR-21-5p, and SOCS6.
Main Results:
- NBAT1 expression is downregulated in BC tissues and correlates with higher pathological grade and metastasis.
- NBAT1 upregulation inhibits BC cell viability and invasiveness, inducing G1 cell cycle arrest.
- NBAT1 targets miR-21-5p, which in turn targets SOCS6, mediating NBAT1's effects on BC cell phenotypes.
Conclusions:
- Reduced NBAT1 expression is linked to BC progression and metastasis.
- NBAT1 exerts tumor-suppressive effects in BC via the miR-21-5p/SOCS6 pathway.
- The NBAT1/miR-21-5p/SOCS6 axis represents a potential therapeutic target for bladder cancer.
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